| 摘要: |
| 为探究火焰蝶兰群体表型性状遗传变异规律及花色表型多样性，以蝴蝶兰‘聚宝红玫瑰’与云南火焰兰属间杂交F1代其中的117个单株为试验材料，采用性状离散特征描述、Pearson相关性分析及类平均聚类分析统计学等方法，对35个表型性状及花色表型多样性进行分析。结果表明：1）35个表型数量性状变异范围为7.6%~239.1%，变异程度大；性状值分布于双亲的性状之间，且都呈现明显的单峰分布，整体呈现较好的连续正态分布趋势；中亲优势率为-94.54%~1 336.98%，其中叶片长度等15个性状表现为正向中亲优势，株高等20个性状中亲优势值为负值。2）35个性状中分别有281对相关性达到极显著水平（P<0.01），有74对达到显著水平（P<0.05）；系统聚类将117个单株在遗传距离16.10处分为Ⅰ、Ⅱ、Ⅲ这3大类群，聚类结果反映出各类群的性状特征。3）结合RHSCC和CIELab参数值将F1代花色分为浅紫色、紫红色、橙红色、橙色、橙黄色和黄色6色系，各色系参数与CIELab表色空间的明度（L*）、红度（a*）和黄度（b*）均有良好的对应关系。综上，本研究为后续开展重要观赏性状的数量性状基因座定位等研究奠定基础，为兰科植物杂交亲本的选配以及新品种的选育提供参考。 |
| 关键词: 蝴蝶兰 火焰兰 属间杂交 表型分析 |
| DOI：10.11841/j.issn.1007-4333.2024.09.06 |
| 投稿时间：2023-11-08 |
| 基金项目:福建省观赏植物种质资源创新与应用工程技术项目（PTJH16005）；广东省园林花卉种质创新综合利用重点实验室开放课题项目（粤农科环艺［2023］4号）；高端外国专家引进计划项目（G2022030025L）；广东省现代农业产业技术体系创新团队建设项目（花卉）（2023KJ121）；广东省农业科学院学科团队建设项目（202127TD） |
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| Genetic variation analysis of hybrids F1 representative traits between Phalaenopsis Jiuhbao Red Rose and Renanthera imschootiana |
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YANG Songmin1,2, LV Fubing2, JIANG Xiao1, WANG Shunshun1, XIAO Wenfang2, LI Zuo2, CAI Wenjie3, ZHU Genfa2, CHEN Heming2, PENG Donghui1
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| (1.College of Landscape Architecture and Arts/Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Environmental Horticulture Research Institute/Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;3.Institute of Tropical Plant Sciences and Microbiology, Cheng Kung University, Tainan 701, China) |
| Abstract: |
| In order to explore the genetic variation of population phenotypic traits and the diversity of flower and color phenotypes， 117 individual plants of hybrid F1 between Phalaenopsis Jiuhbao Red Rose and Renanthera imschootiana Rolfe were used as experimental materials. Trait discrete character description， Pearson correlation analysis and class average cluster analysis were used. Thirty-five phenotypic traits and diversity of flower and color phenotypes were analyzed. The results showed that： 1） The variation of 35 phenotypic quantitative traits ranged from 7.6% to 239.1%， with a large degree of variation. The trait values were distributed between the traits of both parents with an obvious unimodal distribution， and the overall trend of continuous normal distribution was good. The ratio of mesophilic dominance ranged from -94.54% to 1 336.98%. Among them， 15 traits such as leaf length showed positive mesophilic dominance， while 20 traits of plant height showed negative parental dominance. 2） There were 281 pairs of highly significant correlations （P<0.01） and 74 pairs of significant correlations （P<0.05） among the 35 traits. By systematic clustering， the 117 individual plants were divided into three groups， e.g.Ⅰ， Ⅱ and Ⅲ， at the genetic distance of 16.10， and the clustering results reflected the character characteristics of each group. 3） Combining RHSCC and CIELab parameters， the colors of F1 generation were divided into six color groups， which were light purple， fuchsia， orange red， orange， orange yellow and yellow. The parameters of each color group had a good correspondence with the lightness （L*）， redness （a*） and yellow （b*） of the CIELab surface color space. In summary， this study laid the foundation for further research on quantitative trait loci mapping of important ornamental traits， and provided a reference for the selection of hybrid parents and the breeding of new varieties of Orchidaceae. |
| Key words: Phalaenopsis spp. Renanthera intergeneric hybridization trait analysis |
