| 摘要: |
| 为明确软腐病害生理机制，提高甘薯贮藏期的品质，以浙江地区甘薯主要鲜食品种‘心香’为试验材料，对其病菌侵染前后的生理生化响应和转录组学进行分析。结果表明：1）软腐病侵染后，甘薯抗氧化酶和细胞壁降解酶活性显著提高，超微结构显示甘薯细胞结构受到严重破坏。2）甘薯软腐病侵染过程中有12 205个基因发生显著变化，其中7 505个上调表达，4 700个下调表达；GO类目中，差异表达基因（DEGs）被富集到细胞器、膜等细胞部位，代谢过程、生物活性和相应刺激反应等生物过程，催化活性等分子功能。KEGG注释分析可知，DEGs显著富集在苯丙烷代谢、碳代谢、淀粉蔗糖代谢、氨基酸生物合成、柠檬酸循环和激素信号转导次生代谢产物的生物合成中。本研究为进一步甘薯抗病相关基因功能的研究和抗病机理的解析提供了理论参考。 |
| 关键词: 甘薯 软腐病 超微结构 转录组 |
| DOI：10.11841/j.issn.1007-4333.2024.09.05 |
| 投稿时间：2024-01-12 |
| 基金项目:国家自然科学基金项目（32101650）；财政部和农业农村部国家现代农业产业技术体系资助项目（CARS-10-GW21） |
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| Physiological and biochemical responses and transcriptome analysis of sweet potato under soft rot fungus stress |
|
AN Yanbo1, ZHANG Liang2, LIU Penghui1, MA Wenqing1, LU Guoquan1, CUI Peng1
|
| (1.College of Advanced Agricultural Science, Zhejiang A&F University, Hangzhou 311300, China;2.Jinhua Academy of Agricultural Sciences, Jinhua 321000, China) |
| Abstract: |
| In order to clarify the physiological mechanism of soft rot disease and improve the quality of sweet potato during storage， the physiological and biochemical responses and transcriptomics of “Xinxiang”， the major fresh eating variety of sweet potato in Zhejiang， were analyzed before and after disease infection. The results showed that： 1） After infection with soft rot disease， the activities of antioxidant enzymes and cell wall-degrading enzymes in sweet potato significantly increased， and the ultrastructure analysis showed severe damage to the cell structure. 2） During the process of sweet potato soft rot disease infection， 12 205 genes showed significant changes， among which， 7 505 displayed upregulated expression and 4 700 displayed downregulated expression； In the GO category， different expression genes were enriched in organelles， membranes and other cellular components， metabolic processes， biological activities， response to corresponding stimuli and catalytic activities in molecular functions. KEGG annotation analysis revealed that DEGs were significantly enriched in phenylpropane metabolism， carbon metabolism， starch and sucrose metabolism， amino acid biosynthesis， citric acid cycle， hormone signaling transduction and biosynthesis of secondary metabolites. This study provided a theoretical reference for further research on the gene function related to sweet potato disease resistance and the elucidation of disease resistance mechanisms. |
| Key words: sweet potato soft rot ultrastructure transcriptome |
