摘要: |
为探究马铃薯转录因子StNAC043基因的功能,克隆马铃薯转录因子StNAC043基因,利用农杆菌介导法将其转入马铃薯‘东农310’基因组,使其过量表达。转基因植株经无性系扩繁后,测定转基因株系的根和茎的生长指标、叶绿素相对含量(SPAD)以及荧光参数(Fv/Fm、ETRmax),并以转录组测序结合qRT-PCR验证分析其下游调控基因。结果表明:StNAC043基因过量表达可以显著促进转基因马铃薯根系和茎的生长、显著提高转基因植株叶片SPAD、Fv/Fm、ETRmax;并且转录因子StNAC043可以调控捕光色素结合蛋白基因StCAB1和StCAB2的表达。综上,马铃薯转录因子StNAC043基因对马铃薯幼苗的生长和光合生理调控具有重要作用。 |
关键词: 马铃薯 转录因子 StNAC043 根系 荧光参数 |
DOI:10.11841/j.issn.1007-4333.2023.10.06 |
投稿时间:2023-01-03 |
基金项目:现代农业产业技术体系建设专项(CARS-09);寒地粮食作物种质创新与生理生态教育部重点实验室开放课题(CXSTOP202205) |
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Cloning and physiological function analysis of potato transcription factor StNAC043 gene |
TANG Xinhua1,2,QU Zicheng1,LI Shiwei1,LIU Yulin1,SHI Ying1*
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(1.Agricultural College, Northeast Agricultural University, Harbin 150030, China;2.National Research Center of Soybean Engineering and Technology, Harbin 150028, China) |
Abstract: |
To reveal the function of potato transcription factor StNAC043 gene, a potato transcription factor StNAC043 gene was cloned and transferred into potato ‘Dongnong 310' genome by agrobacterium-mediated method to overexpress the gene. After clonal propagation of transgenic plants, the root and stem growth indexes, chlorophyll relative content(SPAD)and fluorescence parameters(Fv/Fm and ETRmax)of transgenic lines were measured, and the downstream regulatory genes were verified by transcriptome sequencing combined with qRT-PCR. The results showed that the overexpression of StNAC043 gene significantly promoted the growth of roots and stems of transgenic potato, significantly increased the SPAD, Fv/Fm and ETRmax of transgenic plants, and the transcription factor StNAC043 regulated the expression of light-harvesting pigment binding protein genes StCAB1 and StCAB2. In conclusion, the potato transcription factor StNAC043 gene plays an important role in potato seedling growth and photosynthetic physiological regulation. |
Key words: potato transcription factor StNAC043 root fluorescence parameters |