摘要: |
使用中性蛋白酶、碱性蛋白酶、胰蛋白酶、风味蛋白酶和木瓜蛋白酶对羊乳清蛋白进行水解,分别在不同时间取样,体外测定其ACE抑制活性和DPP-IV抑制活性。通过中空纤维膜超滤、凝胶过滤层析和反向高效液相色谱(RP-HPLC)的手段对酶解产物进行逐步分离和纯化,评价各级分离产物的生物活性;通过液质联用(LC-MS/MS)的方法鉴定多肽的氨基酸组成,根据质谱结果合成多肽。结果表明:中性蛋白酶水解物具有一定的生物活性,双重活性多肽Pro-Pro-Ala-Ser-Glu-Val-Val-Lys-Pro对ACE和DPP-IV均有抑制作用,其IC50值分别是573.32±4.63和2 065.35±22.30 μmol/L。Ile-Pro-Ala-Val-Phe-Lys-Ile-Asp具有较好的DPP-IV抑制活性,其IC50值为964.14±4.09 μmol/L。羊乳清蛋白水解物可以作为食源性材料用于管理伴有高血压的Ⅱ型糖尿病患者的饮食。 |
关键词: 羊乳清蛋白 ACE抑制活性 DPP-IV抑制活性 双重活性多肽 |
DOI:10.11841/j.issn.1007-4333.2018.12.12 |
投稿时间:2018-04-16 |
基金项目:国家重点研发计划项目(2017YFD0400200) |
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Purification and identification of angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory peptides from goat whey protein hydrolysates |
ZHANG Yuqi1, GAO Song1, LI Qian1, ZHANG Yanchun2, DAI Zhiyong2, LUO Yongkang1
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(1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Ausnutria Dairy(China) Co. Ltd., Changsha 410200, China) |
Abstract: |
Goat whey protein was hydrolyzed with neutrase, alcalase, trypsin, flavourzyme and papain. The hydrolysates were sampled at different time points. ACE and DPP-IV inhibitory activities were measured at the concentration of 5 mg/mL. The neutrase-generated hydrolysate was isolated by ultrafiltration, gel filtration chromatography, reversed-phase high-performance liquid chromatography (RP-HPLC) and then identified via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The peptides were synthesized and assayed for bioactivities. The results showed that the hydrolysate treated with neutrase showed certain bioactivities. The dual bioactive peptide Pro-Pro-Ala-Ser-Glu-Val-Val-Lys-Pro showed inhibition against ACE and DPP-IV with IC50 values of 573.32±4.63 and 2 065.35±22.30 μ mol/L, respectively. Ile-Pro-Ala-Val-Phe-Lys-Ile-Asp had better DPP-IV inhibitory activity with an IC50 value of 964.14±4.09 μ mol/L. In conclusion, the goat whey protein hydrolysates can be used as a food-derived material for managing the diet of type 2 diabetes mellitus patients with hypertension. |
Key words: goat whey protein angiotensin-converting enzyme dipeptidyl peptidase IV dual bioactive peptide |