摘要: |
为了解EPS的粒径、热稳定性、乳化特性及流变学特性,以实验室选育的一株胞外多糖(EPS)高产菌株(Bacillus amyloliquefaciens LPL061)为材料,并考察EPS的体外免疫调节功能。结果表明:芽孢杆菌EPS粒径大小为445.6 nm;熔融温度为265.18 ℃;对食用油乳化效果较好,乳化指数达50%以上;EPS溶液为典型的假塑性流体,在20~80 ℃范围内黏度变化较小,具有较好耐温性;EPS能促进小鼠脾淋巴细胞增殖及细胞因子产生,具有一定的免疫活性。 |
关键词: 解淀粉芽孢杆菌 胞外多糖 理化特性 免疫调节 |
DOI:10.11841/j.issn.1007-4333.2014.01.18 |
投稿时间:2013-04-30 |
基金项目:公益性行业(农业)科研专项经费项目(201303014-4);国家自然科学基金资助项目(31271827);国家大学生创新性实验计划项目(201210019088) |
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Physicochemical properties and immunomodulatory effects of exopolysaccharide produced by Bacillus amyloliquefaciens |
FAN Yi, LIU Li-sha, HAN Yu-zhu, LI Ping-lan
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(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) |
Abstract: |
In this study,the particle size,thermal stability,rheological properties,emulsifying properties and immunomodulatory effects of exopolysaccharide of a high-yield strain Bacillus amyloliquefaciens LPL061 were studied and compared with those of xanthan gum.The results showed that the molecular size of EPS was 445.6 nm,the melting temperature is 265.18 ℃.LPL061 produced more stable emulsions with edible oils than xanthan gum.EPS also showed pseudoplastic non-Newtonian fluid behaviour in an aqueous solution,with thermal stability between 20 to 80 ℃.The EPS from LPL061 showed potential of immunomodulatory effects by promoted the lymphocyte proliferation of mouse. |
Key words: Bacillus amyloliquefaciens Exopolysaccharides(EPS) physicochemical properties immunomodulatory |