摘要: |
为了弄清DA-6对不同光强下草莓光合作用提高的效果,以'甜查理’草莓为试材,通过遮荫处理,研究了100%、70%和50%自然光条件下喷施20 mg/L DA-6处理对草莓叶片气体交换参数、光合酶活性及抗氧化酶活性的影响。结果表明:遮荫条件下,草莓叶片净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、核酮糖-1,5-二磷酸加氧/羧化酶(Rubisco)、果糖-1,6-二磷酸酯酶(FDPase)活性均明显降低,而胞间CO2浓度(Ci)升高。表明遮荫条件下,草莓叶片光合速率下降的主要原因是非气孔限制,而且与光合相关酶类活性下降密切相关。DA-6处理可以不同程度提高各光强条件下草莓叶片的Pn、Gs、Tr及Rubisco、FDPase的活性,同时降低超氧阴离子(O-2)产生速率,提高SOD、CAT和APX等保护酶的活性,从而提草莓叶片的光合作用。在相对较弱的光强条件下(50%自然光),DA-6的作用更为显著。 |
关键词: 草莓 光强 DA-6 光合作用 抗氧化酶 |
DOI:10.11841/j.issn.1007-4333.2011.05.013 |
投稿时间:2011-03-09 |
基金项目:公益性行业(农业)科研专项(3-37) |
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Effects of DA-6 on leaf photosynthesis and antioxidant enzymeactivities of strawberry under different light intensities |
LIANG Yan-ping1, MU Hong-mei1, WANG Jing-jing1, LIU Guo-jie1, Saimaiti·yusufu2
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(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;2.Kuche Forestry Bureau of Aksu Prefecture,Akesu 842000,China) |
Abstract: |
In order to elucidate the regulation effects of DA-6 on the photosynthesis of strawberry under different light intensities,DA-6 of 20 mg/L,was sprayed on the leaves of the seedlings of 'Sweet Charlie’ strawberry under 100%,70% and 50% sunlight.The leaf gas exchange parameters,activities of Rubisco and FDPase,antioxidant enzyme activities were measured.The results showed that under shade condition,the leaf photosynthetic rate (Pn),stomatal conductance (Gs),transpiration rate(Tr),Rubisco and FDPase activities decreased,but the intercellular CO2 concentration (Ci) increased.It is thus clear that the decrease of Pn under low light intensity is mainly due to the non stomatal limitation and closely related to the decrease of Rubisco and FDPase.However,foliar spraying of DA-6 increased the leaf Pn,Gs,Tr and activities of Rubisco,FDPase,SOD,CAT and APX,but reduced the O2-production rate. The improvement of photosynthesis was much more significant under 50% sunlight. |
Key words: strawberry light intensities DA-6 photosynthesis antioxidant enzyme activities |