摘要: |
以抱子甘蓝和羽衣甘蓝为材料,采用常规压片法进行核型分析,结果表明:抱子甘蓝的核型公式为2n=2x=18=10+8sm(2SAT),其中第3、4、5、6、8对为中着丝粒染色体,第1、2、7、9对为近中着丝粒染色体,第7对染色体具有随体;相对长度组成为2n=2L+6M2+10M1 ;核型分类为2A型,属于基本对称型。羽衣甘蓝的核型公式为,2n=2x=18=10m+8sm,其中第3、4、6、7、8对为中着丝粒染色体,第1、2、5、9对为近中着丝粒染色体,未观察到随体染色体;染色体相对长度组成为2n=8M2+10M1;核型分类为2A型,属于基本对称型。 |
关键词: 抱子甘蓝 羽衣甘蓝 染色体 核型分析 |
DOI:10.11841/j.issn.1007-4333.2010.02.002 |
投稿时间:2009-08-05 |
基金项目:江苏省自然科学基金项目(SBK20082174)第一作者:张红梅,硕士研究生,E-mail:zhanghongmei1214@163.com通讯作者:张蜀宁,副教授,硕士生导师,主要从事园艺植物多倍体遗传育种研究,E-mail: snzhang@njau.edu.cn |
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Chromosome karyotype analysis of brussels sprouts and collard |
ZHANG Hong-mei1,2, ZHANG Shu-ning1,2, YU Xu-hong1,2, ZHENG Jin-shuang1,2
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(1. College of Horticulture/Key Laboratory of Southern Vegetable Crop Genetics Improvement of Ministey of Agriculture, Nanjing Agricultural University, Nanjing 210095,China;2. Tianjin Song Jiang Ecology Industry Co.,Ltd., Tianjin 300385, China) |
Abstract: |
The karyotypes of brussels sprouts and collard were studied by using traditional squash method. The results showed that the karyotype of Brussels sprouts is 2n=2x=18=8m+10sm (2SAT), with 5 pairs (No. 3,4,5,6,8) metacentricand, 4 pairs (No. 1,2,7,9) acrocentric chromosomes. Satellites were found at the seventh pair of the chromosomes. Constitution of relation length was 2n=2L+6M2+10M1. Characterized as 2A karyotype, Brussels sprouts presented an ultimately symmetry karyotype. Similarly, the karyotype of Collard is 2n=2x=18=10m+8sm, with 4 pairs metacentric (No. 3,4,6,7,8) and 5 pairs acrocentric (No. 1,2,5,9) chromosomes, but no satellite was observed. Constitution of relation length was 2n=8M2+10M1. As a 2A karyotype, collard presented an ultimately symmetry karyotype. |
Key words: brussels sprouts collard chromosome karyotype analysis |