摘要: |
以杜仲一叶一心期幼叶为材料,提取总BNA,采用RT-PCR技术分离得到498bp核苷酸片段,此核苷酸片段与CenBank中的苹果树肉桂醇脱氢酶(CAD)基因序列有64.1%的同源性,与桉树mRNA CAD有63.9%的同源性。所克隆的cDND编码165个氨基酸残基,其序列与苹果树CAD相应片段有68.5%的同源性,与桉树mRNA CAD相应片段有66.1%的同源性。推测克隆的核香酸片段为杜仲CAD的基因片段。 |
关键词: 杜仲 肉桂醇脱氢酶 基因克隆 序列分析 同源性 木质素 |
DOI:10.11841/j.issn.1007-4333.2003.01.009 |
修订日期:2002-06-04 |
基金项目:国家自然科学基金资助项目 (39870 6 4 4 ),农业生物技术国家重点实验室基金开放课题资助项目 (9910 0 4 2 ) |
|
Cloning of cDNA encoding cinnamyl alcohol dehydrogenase from Eucommia ulmoides Oliv |
|
|
Abstract: |
Cinnamyl alcohol dehydrogenase catalyses the conversion of p hydroxy cinnamaldehydes to the corresponding alcohols and is considered as a key enzyme in lignin biosynthesis. In this study, a cDNA fragment of cinnamyl alcohol dehydrogenase (CAD) was cloned from Eucommia ulmoides Oliv by RT PCR. As deduced from the 498 bp cDNA nucleotide sequence, it presented 68.5% identity with Malus domestica putative CAD and 66.1% identity with E.gunnii mRNA for CAD. These results suggest that the cDNA fragment is from CAD gene of Eucommia ulmoides Oliv. |
Key words: Eucommia ulmoides Oliv,cinnamyl alcohol dehydrogenase,lignin,DNA sequence |