摘要: |
amy-3是枯草芽孢杆菌的一个基因突变类型,它造成产淀粉酶的表型丧失。本实验初步证明,克隆的 sacQ 基因转入 amy-3枯草芽孢杆菌菌株 SO113后,可以恢复其淀粉酶活性。表明这个突变没有使淀粉酶结构基因发生改变,突变可能发生在调控基因。 |
关键词: 枯草芽孢杆菌 amy-3 sacQ 基因 |
DOI: |
投稿时间:1994-11-24 |
基金项目: |
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Evidence of amy-3 Mutation of Bacillus subtilis SO113 Caused by sacQ Gene Inactivation |
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Abstract: |
amy-3 is a mutation of B.sabtilis which causes the deletion of production of amylase on B. subtilis strain SO113.It was found that this phenotype is due to inactivation of sacQ gene rather than the deletion of amylase structure gene.Transformation of SO113 with cloned sacQ could rescue the phenotype of production of amylase. |
Key words: B.subtilis amy-3 sacQ gene |